IR820 Dibenzocyclooctyne

IR820 Dibenzocyclooctyne

 290.00 1856.00

For Cu-free click-chemistry labeling

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Introduction

IR820 is a non-invasive near-infrared (NIR) fluorescence imaging dye that belongs to the family of the heptamethine dyes and has a rigid cyclohexenyl ring, increasing molecular stability and photostability. IR820 could be used as both an imaging dye and a hyperthermia agent.1

IR820 has a little absorption in the visible range thus exhibit low autofluorescence, tissue absorbance, and scatter at NIR wavelengths (700-900 nm).

 

Dibenzocyclooctyne derivative of the IR820 dye offer the opportunity to develop optimal conjugates. Dibenzocyclooctyne reactive groups provide an efficient and convenient way to selectively link IR820 dyes to azides on various substrates (Antibodies, peptides, proteins, nucleic-acid, fatty acids, small molecule drug…). Dibenzocyclooctyne are very reactive against azides. The conjugation is based on Huisgen cycloaddition of an azide to strained cyclic alkyne (dibenzocyclooctyne). Unlike traditional azide/alkine reaction it does not require Cu(I) catalyst. Dibenzocyclooctyne are non-endogeneous functional groups, thus they are not naturally present on targeting molecules (biomolecules). On the other hand, azides derivatives of biomolecules and small molecules are widely in research and are commercially available from numerous sources. The reaction proceeds under aqueous conditions at physiological temperatures and pressures. The dibenzocyclooctyne/azide combination is thus a bioorthogonal reaction.

Material Amount Storage Stability
IR820-dibenzocyclooctyne 1 mg
  • -20° C
  • Desiccate
  • Protect from light
When stored as directed, reactive probes are stable for at least 3 months
5 mg
10 mg

Immediately before use, dissolve the IR820-Dibenzocyclooctyne dye in anhydrous dimethylsulfoxide (DMSO).

Conjugate Purification

Labeled antibodies are typically separated from free IR820 dye using a gel filtration column, such as SephadexTM G-25, BioGel® P-30, or equivalent. For much larger or smaller proteins, select a gel filtration media with an appropriate molecular weight cut-off or purify by dialysis.

  1. Fernandez-Fernandez A., Manchanda R., Lei T., Carvajal D.A., Tang Y., Kazmi S.Z.R., and McGoron A.J., Comparative Study of the Optical and Heat Generation Properties of IR820 and Indocyanine Green, Molecular Imaging, 2012, 11, 99–113
Color

Dark brown powder

Label

Cyanobenzothiazole

Product Size

1 kit

Detection Method

Fluorescence, optoacoustic

Excitation Class

Near infrared, NIR

Molecular Weight

967.23 g.mol-1

Excitation/Emission maximum (nm)

710/820

Formula

C84H94N5O15S2

Shipping Condition

RT

Regulatory Statement

For Research Use Only. Not for use in diagnostic procedures.

Quantity

1 mg, 5 mg, 10 mg

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